FIG. 5.
IFN-γ production by LLO-specific CD8+ T cells. Bulk splenocytes were purified from individual mice in groups and restimulated in vitro with LLO91-99 in the presence of brefeldin A. The frequency (A) and absolute number (B) of IFN-γ-positive CD8+ T cells are shown. (C) Representative dot plots showing the IFN-γ levels produced by CD8+ T cells taken from mice before challenge (top panels) and after challenge (bottom panels) with Listeria. Data for all mice analyzed in this way before challenge (open bars) and after challenge (solid bars) are shown in panel D. The bars indicate IFN-γ production expressed as the mean fluorescence intensity (MFI), and the error bars indicate standard deviations (four to six mice per group). Data were analyzed using Student's t test. Vacc, vaccination; L.don, L. donovani.