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The role of Fur in V. cholerae autoagglutination. Strains were inoculated into LB broth at pH 6.5 and grown on a rolling shaker at 30°C for 20 h. (A) O395 (wild type), ARM573 (furΔ::tmp), ARM573/pWKS30 (vector control), ARM573/pAMF1 (complementing fur plasmid), and ARM574 (wild-type fur allelic replacement derivative of ARM573). (B) O395 (wild type), ARM572 (ryhBΔ::kan), ARM573 (furΔ::tmp), ARM572/pQE-2 (vector control), and ARM572/pAMR70 (IPTG-inducible ryhB expression construct).
