TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| V. cholerae strains | ||
| O395 | V. cholerae classical biotype | 46 |
| ARM572 | O395 ryhBΔ::kan | 47 |
| ARM573 | O395 furΔ::tmp | 47 |
| N16961 | V. cholerae EI Tor biotype | J. Kaper |
| E. coli strains | ||
| DH5α | Cloning strain | 28 |
| DH5α(λpir) | Host strain for pHM5 derivatives | J. Kaper |
| Plasmids | ||
| pHM5 | Suicide vector carrying sacB; Cbr Sucs | 64 |
| pQE-2 | IPTG-inducible expression vector | QIAGEN |
| pWKS30 | Low-copy cloning vector; Cbr | 75 |
| pAMF1 | V. cholerae fur in pWKS30 | This study |
| pAMR70 | V. cholerae ryhB in pQE-2 | 47 |
| pAMS17 | V. cholerae fur in pHM5 | This study |