TABLE 6.

Genes within the large integron and the tcp region positively regulated by Fur

ORFa	Gene	Function	fur/WTb	Low iron/high ironc
Large integron
VCA0444	relE	Toxin of toxin-antitoxin stress response locus, COG2026	0.48	0.83
VCA0445		Antitoxin of toxin-antitoxin stress response locus, COG2161	0.37	0.92
VCA0446		Hemagglutinin	0.43	0.91
VCA0451		Hypothetical	0.58	1.1
VCA0453		Hypothetical	0.56	1.1
VCA0455		Hypothetical, COG0693	0.63	0.94
VCA0457		Hypothetical	0.45	1.2
VCA0458		Hypothetical	0.39	0.96
VCA0459		Hypothetical	0.46	1.2
VCA0462		Hypothetical	0.74	1.1
VCA0466		Hypothetical	0.43	NDe
VCA0468		Hypothetical, COG4737	0.41	0.77
VCA0469		HLH_XRE transcriptional regulator family, COG2944	0.38	0.95
VCA0470		Putative acetyltransferase, COG0456	0.47	1.1
VCA0471		Hypothetical	0.54	0.90
TCP regiond
VC0819	aldA-1		0.44	1.2
VC0820	tagA		0.38	0.81
VC0821		Hypothetical	0.51	0.76
VC0822		Putative inner membrane	0.51	0.97
VC0823		Hypothetical	0.60	0.70
VC0824	tagD		0.53	0.56
VC0825	tcpI		0.48	0.93
VC0829	tcpB		0.45	0.76
VC0830	tcpQ		0.49	1.5
VC0831	tcpC		0.47	1.5
VC0832	tcpR		0.45	1.7
VC0833	tcpD		0.44	1.8
VC0834	tcpS		0.43	ND
VC0835	tcpT		0.57	1.5
VC0836	tcpE		0.43	1.2
VC0837	tcpF		0.22	1.0
VC0840	acfB		0.58	1.3
VC0841	acfC		0.55	1.3
VC0842		Hypothetical, COG1714	0.52	1.6
VC0843	tagE		0.54	1.1
VC0844	acfA		0.40	1.1
VC0845		Hypothetical	0.56	0.73
VC0846		Integrase, degenerate	0.75	1.0

^aORF designation from TIGR database (33).

^bMean expression ratio of fur mutant relative to wild-type parental strain O395.

^cMean expression ratio of wild-type strain O395 grown in the absence relative to the presence of iron supplementation.

^dFor a review of individual genes in the tcp/acf cluster, see reference 19.

^eND, not determined.