FIG. 5.

Ultrastructural localization of L3 parasite proteins recognized by antibodies from wild-type mice (A and B) or TLR-4 mutant mice (C and D) immunized with radiation-attenuated L3 and challenged with live L3 as determined by immunogold labeling. Sera from wild-type and TLR4-mutant mice have a similar pattern of recognition of the larvae, including the cuticle (cu) and the channels connecting the pseudocoelum to the cuticles. Note that the Wolbachia (arrowhead) is not labeled by either immune serum sample. In comparison, the Wolbachia (arrowhead) is labeled specifically with anti-WSP antibodies (E). Bar, 0.5 nm.