TABLE 2.
Primers used for construction of TEM-1 β-lactamase fusion proteins
| Name | Sequencea | Site(s) engineered |
|---|---|---|
| Flag-F | ACATATGCTCGAGGACTACAAAGACCATGACGGTG | NdeI, XhoI |
| Flag-R | ACCCGGGTTTATCGTCGTCATCTTTGTAG | SmaI |
| TEM-F | ACCCGGGCACCCAGAAACGCTGGTGAAAG | SmaI |
| TEM-R | ACTGCAGTTATCTAGACCAATGCTTAATCAGTGAG | PstI-Stop-Xbal |
| LaclQ-F | ATCACCGAAACGCGCGAG | |
| Kan-R | ACAACGTCGAGCACAGC | |
| Trc-R | ACCCATATGGTCTGTTTCCTGTGTG | NdeI |
| SipA-F | ACATATGGTTACAAGTGTAAGGAC | NdeI |
| SipA-R | AGTCGACACGCTGCATGTGCAAGCCATC | SalI |
| SopD-F | ACGCTCGAGTAATTGAAGGAAAATATTATATGCCAG | XhoI, Stop, SDb |
| SopD-R | ACGCTCGAGTGTCAGTAATATATTACGACTGC | XhoI |
| GST-F | TCCATATGTCTGAGTCCCCTATACTAGGTTATTG | NdeI |
| GST-R | AACTGCAGTCAACGCGGAACCAGATCCGATTTTG |
a
Restriction sites are underlined.
b
SD, Shine-Dalgarno sequence.