TABLE 1.
Oligonucleotide primers and probes used in PCRs and hybridization assays
| Oligonucleotide primer or probe | Sequence | Target organism | Target gene | Nucleotide position (range) | Reference or source |
|---|---|---|---|---|---|
| Primers | |||||
| 16S8FE | GGAATTCAGAGTTGGATCMTGGYTCAG | Eubacteria | 16S rRNA gene | 8-27 | L. M. Schouls |
| B-GA1B | 5′biotin-CGGGATCCCGAGTTTGCCGGGACTTCTTCT | Anaplasma/Ehrlichia genus | 16S rRNA gene | 476-456 | L. M. Schouls |
| 44k-start | ATGATGTCTATGGCTATAGTCATG | A. phagocytophila group | 44-kDa genea | 49-72 | This study |
| 44k-end | ATCTCCCACAACACGATG | A. phagocytophila group | 44-kDa genea | 1180-1197 | This study |
| 16SEhrSeq | GGAATTCAGAGTTTGATCMTGGYTCAGAAC | Eubacteria | 16S rRNA gene | 8-30 | This study |
| GA1BSeq | CGGGATCCCGAGTTTGCCGGGACTTCTTCTGTA | Anaplasma/Ehrlichia genus | 16S rRNA gene | 476-459 | This study |
| Probes | |||||
| A-EhrA11 | 5′ amino-TTATCGCTATTAGATGAGCC | Anaplasma/Ehrlichia genus | 16S rRNA gene | 203-222 | L. M. Schouls |
| A-HGE | 5′ amino-GCTATAAAGAATAGTTAGTGG | A. phagocytophila prototype | 16S rRNA gene | 87-107 | L. M. Schouls |
| A-Phago | 5′ amino-TTGCTATAAAGAATAATTAGTGG | A. phagocytophila variant 1 | 16S rRNA gene | 85-107 | L. M. Schouls |
| A-Phagovar2 | 5′ amino-GAACGGATTATTCTTTGTAGC | A. phagocytophila variant 2 | 16S rRNA gene | 87-106 | This study |
| A-D-Phago | 5′ amino-TTGCTATGAAGAATAATTAGTG | A. phagocytophila variant 3 | 16S rRNA gene | 87-106 | L. M. Schouls |
| A-D-HGE | 5′ amino-GCTATGAAGAATAGTTAGTG | A. phagocytophila variant 4 | 16S rRNA gene | 87-106 | L. M. Schouls |
| A-Eschot | 5′ amino-GCTGTAGTTTACTATGGGTA | Ehrlichia sp., Schotti variant | 16S rRNA gene | 76-95 | L. M. Schouls |
| A-Ecan | 5′ amino-TCTGGCTATAGGAAATTGTTA | E. canis | 16S rRNA gene | 85-105 | L. M. Schouls |
| A-Echaf | 5′ amino-ACCTTTTGGTTATAAATAATTGTTA | E. chaffeensis | 16S rRNA gene | 83-107 | L. M. Schouls |
| A-EmurisC | 5′ amino-GCTATAGGTTCGCTATTAG | E. muris | 16S rRNA gene | 85-103 | A. N. Alekseev |
| A-TmpBEhr | 5′ amino-GAAAACTCGAAGAACAAAGAA | T. pallidumb | tmpB | 502-522 | A. N. Alekseev |