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. 2002 Sep;40(9):3470–3475. doi: 10.1128/JCM.40.9.3470-3475.2002

FIG. 3.

FIG. 3.

Toxinotyping of eight C. difficile strains with variant PaLoc genes and of the reference strains C. difficile VPI 10463, C. difficile 51377, and C. difficile 57267. (For descriptions of the other toxinotypes, see Rupnik et al. [21, 22]). The PCR-RFLP patterns of A3 and B1 fragments are shown for each strain. A3 fragments were digested with EcoRI (E), and B1 fragments were digested with HincII (H) and AccI (A). M, 100-bp DNA ladder (BioLabs). Pd7, Pd13, Pd16, and Pd55 (C. difficile strains), toxinotype V; Pd5 and Pd53, toxinotype VI; Pd3 and M7, toxinotype 0 (C. difficile M7 represents a new toxinotype, as demonstrated by PCR-RFLP analysis of the entire PaLoc). C. difficile VPI 10463, C. difficile 51377, and C. difficile 57267 are the reference strains for toxinotypes 0, VI, and VII, respectively.