Agarose gel analysis of PCR amplification products of 16S-tRNAIle region of eight California (C) and four Iowa (I) PDD-associated Treponema isolates. Lanes: 1, 1-9185MED; 2, 9-3143; 3, 9-3528; 4, 9-3379; 5, 9-227; 6, 2-1498; 7, 7-2009; 8, 9-3301; 9, 1A; 10, 3A; 11, 4A; and 12, 5B. The positions of size standards (in base pairs) are indicated on the left side of the gel.