TABLE 2.
Comparison of 16S-23S rDNA ISRs of six California PDD-associated Treponema isolates and other spirochetes
| Spirochete | Copy no. of 16S-23S rRNA genesa | ISR length (nucleotides) | tRNA gene(s) in ISR |
|---|---|---|---|
| PDD 1-9185MED | 2 | 452/449 | Ala or Ile |
| PDD 9-3143 | 2 | 454/450 | Ala or Ile |
| PDD 9-3528 | 2 | 454/450 | Ala or Ile |
| PDD 9-3379 | 2 | 367/368 | Ala or Ile |
| PDD 2-1498 | 2 | 380/373 | Ala or Ile |
| PDD 7-2009 | 2 | 380/373 | Ala or Ile |
| T. denticola | 2 | 400/383 | Ala or Ile |
| T. medium | 2 | 365/369 | Ala or Ile |
| T. vincentii | 1 | 405 | Ala |
| T. phagedenis | 2 | 384/374 | Ala or Ile |
| T. pallidum | 2 | 302/292 | Ala or Ile |
| B. burgdorferi | 1b | 3051 | Ala and Ile |
| B. hermsii | 1c | 5854 | Ala and Ile |
| Leptonema illini | 2 | 435/NDd | None/ND |
| Leptospira biflexa | None | NAe | NA |
| Leptospira interrogans | None | NA | NA |
| Brachyspira hyodysenteriae | None | NA | NA |
a
Copy number of linked 16S-23S rRNA genes based on cloning, Southern hybridization, and/or genomic sequence data.
b
Unusual arrangement (16S-ISR-23S-5S-23S-5S). ISR contains a tRNAAla gene, a tRNAIle gene, and two open reading frames.
c
ISR contains a tRNAAla gene, a tRNAIle gene, and 5 open reading frames.
d
ND, not determined.
e
NA, not applicable.