TABLE 2.
Comparison of CLA susceptibility of H. pylori strains and genotypic detection of mutations associated with CLA resistance by DG-DGGE analysis of gastric biopsy samples obtained from HIV-seropositive and -seronegative patients with dyspeptic symptoms
| Patients | No. of samples | No. histology positive | Phenotype analysis
|
Genotype analysis
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| No. culture positive | No. (%) of samples
|
No. PCR positive | No. (%) positive by DG-DGGE analysis
|
||||||
| CLA susceptible | CLA resistant | Wild type | All mutationsa | Mutations at 2143 and 2144 | |||||
| HIV seropositive | 102 | 49 | 37 | 23 (62.2) | 14 (37.8) | 46 | 26 (56.5) | 20 (43.5) | 18 (39.1) |
| HIV seronegative | 38 | 26 | 18 | 17 (94.4) | 1 (5.6) | 23 | 18 (78.3) | 5 (21.7) | 2 (8.7) |
| Total | 140 | 75 | 55 | 40 (72.7) | 15 (27.3) | 69 | 44 (63.8) | 25 (36.2) | 20 (29) |
a
All of the samples with electrophoretic mobility different from that of the CLA-susceptible reference strain are included here.