FIG. 1.

Comparison of Ag capture ELISA, TaqMan, and traditional RT-PCR used to test pools of naturally infected mosquitoes. TaqMan Ct values represent the threshold cycle number at which fluorescence increases above a fixed threshold value. Symbols: ▴, pools positive by TaqMan, Ag capture ELISA, and RT-PCR; ▪, pools positive by TaqMan and Ag capture ELISA but equivocal or negative by RT-PCR; ♦, pool positive by TaqMan and RT-PCR, but negative by Ag capture ELISA; •, pools positive by TaqMan only. ELISA plates were read at 450 nm; all symbols below the dotted line represent negative results by Ag capture ELISA.