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. Author manuscript; available in PMC: 2005 Dec 6.
Published in final edited form as: J Biol Chem. 2005 Sep 12;280(45):38102–38107. doi: 10.1074/jbc.M504342200

Fig. 5.

Fig. 5

Knockdown of VHL expression fails to enhance HIF-1α transcriptional activity in hypoxia. A, VHL siRNA was used in Hep3B cells to silence VHL expression. The loss of VHL expression was shown by Western analysis, in concomitance with the elevation of HIF-1α levels in normoxia. EGLN1 levels were also determined. Endogenous β-actin was used as a loading control. Arrowhead denotes nonspecific detection. B, PGK1 expression in Hep3B cells was analyzed by real-time RT-PCR after transfection with siRNAs as indicated. PGK1 mRNA levels in reference to those of endogenous ACTB are presented in means ± SE.