TABLE 2.
Expression of biotin-related genes in leukocytes of 7 subjects before and after consuming the egg-white diet12
| Relative gene expression
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subject | Day | MCCA | MCCB | PCCA | PCCB | PC | ACCA | ACCB | HCS | Biotinidase | SMVT | SLC19A3 |
| 1 | 0 | 33 | 24 | 52 | 24 | 18 | 26 | 50 | 28 | 28 | 25 | 320 |
| 28 | 20 | 16 | 24 | 17 | 12 | 15 | 28 | 16 | 16 | 15 | 40 | |
| 2 | 0 | 22 | 20 | 25 | 20 | 14 | 19 | 40 | 17 | 22 | 20 | 152 |
| 28 | 17 | 16 | 18 | 17 | 12 | 16 | 34 | 14 | 17 | 16 | 33 | |
| 3 | 0 | 39 | 33 | 44 | 28 | 20 | 28 | 81 | 31 | 41 | 31 | 392 |
| 28 | 23 | 17 | 29 | 19 | 14 | 17 | 60 | 23 | 24 | 18 | 145 | |
| 4 | 0 | 18 | 15 | 20 | 15 | 14 | 15 | 38 | 11 | 18 | 17 | 112 |
| 28 | 17 | 14 | 17 | 15 | 12 | 13 | 41 | 11 | 16 | 12 | 188 | |
| 5 | 0 | 18 | 14 | 15 | 17 | 13 | 14 | 34 | 11 | 16 | 15 | 103 |
| 28 | 18 | 16 | 14 | 17 | 11 | 12 | 30 | 10 | 16 | 15 | 33 | |
| 6 | 0 | 29 | 26 | 25 | 23 | 16 | 21 | 60 | 20 | 26 | 26 | 435 |
| 28 | 25 | 22 | 24 | 24 | 14 | 19 | 48 | 19 | 24 | 25 | 72 | |
| 7 | 0 | 20 | 14 | 15 | 15 | 9 | 12 | 34 | 14 | 15 | 14 | 144 |
| 28 | 18 | 14 | 12 | 12 | 7 | 11 | 30 | 11 | 14 | 12 | 78 | |
Values are means, n = 3, of 18s-normalized RT-PCR data. The overall CV was 5.5 ± 5.9%; the CV for SLC19A3 was 17 ± 11%.
d 0: before consuming egg-white diet; d 28: after consuming egg-white diet.