Skip to main content
. 2005 Dec 8;116(1):70–79. doi: 10.1172/JCI25445

Figure 3.

Figure 3

USAG1–/– mice showed reduced EMT and tubulointerstitial fibrosis in UUO. (A) Representative histology of the obstructed kidney in wild-type mice and USAG1–/– mice 14 days after the operation. Scale bars: 100 μm. (B) Semiquantitative evaluation of morphologic kidney damage in wild-type mice and USAG1–/– mice, expressed as relative severity on a scale from 0 to 4 (n = 6). (C) E-cadherin expression in UUO. Kidney lysates were subjected to immunoblotting with anti–E-cadherin antibody. Representative data from 4 independent experiments are shown. (D) Immunostaining of α-SMA in UUO. Arrowheads indicate vascular smooth muscle cells. (E) Immunostaining of type IV collagen in UUO. Scale bars: 10 μm. (F) Gene expression in UUO. Gene expression was determined by real-time RT-PCR. In each experiment, the expression levels were normalized to the expression of GAPDH and expressed relative to expression in mice on day 0. n = 4–6 for each experiment. #P < 0.01; †P < 0.005; *P < 0.001; **P < 0.0001. Sham ope, mice 14 days after sham operation; day 14, mice 14 days after UUO.