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. 2005 Dec 8;116(1):70–79. doi: 10.1172/JCI25445

Figure 4.

Figure 4

Enhanced BMP signaling in kidneys of USAG1–/– mice. (A) Phosphorylation of Smad1/5/8 in kidneys of USAG1–/– mice and WT mice. The number of pSmad1/5/8-positive nuclei (upper panels) was counted in 10 consecutive fields in each specimen and normalized by the number of DAPI-positive nuclei (lower panels). n = 6. Scale bar: 10 μm. *P < 0.001; **P < 0.0001. Non treat, mice without disease models. (B) Double immunostaining of phospho-Smad1/5/8 and phospho-Smad2/3. Almost all the nuclei positive for pSmad1/5/8 were negative for pSmad2/3. Scale bar: 10 μm. (C) Immunoblotting of phospho-Smad1/5/8 in kidney lysates prior to disease induction and in both kidney disease models. Representative data from 5 independent experiments are shown.