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. 2002 Oct;3(10):975–981. doi: 10.1093/embo-reports/kvf194

Figure 2.

Figure 2

Binding of HP1α to pericentromeric heterochromatin requires both the chromo domain and the hinge. (A) Schematic representation of the HP1α deletion constructs. (B) Purified histones were resolved by SDS–PAGE as in Figure 1C and then incubated with the indicated GST–HP1α constructs as described in Figure 1A. (C–N) Fixed NIH 3T3 cells were incubated with indicated GST–HP1α constructs as described in Figure 1A. Dots observed upon staining with HP1α(67–119) correspond to the nucleoli. DNA was stained with DAPI (I–N). Scale bar: 6 μm.