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. 2002 Oct;3(10):956–961. doi: 10.1093/embo-reports/kvf192

Figure 3.

Figure 3

PNA-mediated phased activity of PleI. Extent of the forward strand nicking for the pD5-MF set (A) or the forward (upper panel; nicking only) and reverse (lower panel; nicking and cutting) strands cleavage for pD5-MR set (B) is plotted against the distance between the PNA-binding site and the 5′-end of the degenerate recognition sequence 5′-TAATC. The extent of the reaction was assessed following 40 min (black) and 80 min (red) incubation with PleI using the protocol described in Methods. Bar deviations in graphs correspond to the standard errors.