Table 1.

PNA-induced activity of PleI depends on the sequence of pseudo-site^a

Degenerate sequence	Fraction (%) of DNA fragmentsb
	Intact	Nickedc	Cutd
AAGTC	14	33	53
TAATC	17	83	−
TAGTC	34	48	18
GAGTT	76	24	−
TAACT	100	−	−
ACTGA	100	−	−

^aDNA constructs were obtained by substituting pseudosite in pD5-9F with sequences similar to 5′-GAGTC (correct PleI recognition sequence). Changes are underlined.

^bStandard error ≤7% (in triplicate experiments).

^cNick is located 4 nt downstream of degenerate sequence on the forward strand.

^dBoth strands are cleaved at the degenerate site.