Table 3.

Analysis of variance (ANOVA) for all biochemical traits under unprimed (UP), hydro-priming (H), and nano-priming (N) conditions (combined ANOVA)

Sources of variance	Genotypes (G)			Treatment (T)			Replicates (R)			T x G			R x G
	UP	H	N	UP	H	N	UP	H	N	UP	H	N	UP	H	N
(Degree of freedom) DF	9			1			2			9			18
Protein (Pro)	147.62**	258.51**	550.35**	145.28**	269.34**	1.97	0.45	0.16	2.84+	42.45**	34.44**	84.67**	1.07	1.05	1.16
Carbohydrates (C)	406.18**	582.56**	1126.91**	1532.87**	170.56**	5092.82**	0.27	0.93	2.22	42.72**	41.19**	157.22**	1.19	0.71	2.43*
Proline (Pr)	286.98**	1288.55**	510.80**	4859.33**	403.38**	3484.71**	0.15	4.84*	2.73+	241.71**	456.97**	325.09**	1.25	2.67*	1.54
Sodium (Na+)	625.78**	717.85**	637.20**	2837.84**	1898.74**	2220.59**	0.62	2.31	3.61*	62.68**	46.16**	81.51**	1.22	1.03	2.17*
Potassium (K+)	491.24**	493.76**	223.11**	146.47**	366.25**	350.50**	0.00	0.61	0.49	200.29**	66.27**	87.94**	1.10	0.48	0.46
Potassium- Sodium ratio (K+/Na+)	157.87**	175.77**	130.65**	35.22**	2.02	2.52	0.00	0.00	2.25	73.73**	36.88**	48.71**	0.75	0.70	0.00
Sodium- Potassium ratio (Na+/K+)	78.07**	227.62**	117.07**	86.89**	8.12**	0.11	0.05	0.59	0.69	57.98**	33.57**	31.14**	0.68	0.62	0.41
Phosphorus (P)	111.57**	429.86**	205.02**	16.87**	232.47**	376.22**	0.87	0.02	1.64	10.92**	32.84**	18.92**	0.43	2.77*	1.64
Peroxidase (POD)	1258.71**	1186.13**	1342.80**	937.57**	106.01**	1935.46**	2.10	0.84	3.07+	293.63**	104.98**	190.50**	1.30	1.36	1.41
Glutathione S Transferase (GST)	248.89**	475.04**	1620.01**	1681.71**	1486.95**	4327.71**	1.26	0.52	0.57	165.79**	89.58**	312.57**	1.08	0.87	2.11+
Catalase (CAT)	355.69**	2374.55**	885.41**	2283.21**	8693.77**	4995.56**	2.19	5.18*	0.57	170.51**	828.87**	374.07**	0.54	5.87**	1.16
Total Antioxidant Capacity (TAC)	741.52**	916.00**	646.43**	685.18**	324.87**	1001.10**	3.18 +	1.93	0.60	133.69**	176.07**	56.18**	2.30*	1.30	1.09

^*significant at P = 0.05 and + significant at P = 0.1

^**Significant at P = 0.01