TABLE 1.
Isolatea | Designation | Source (yr of isolation) | Type of RAPD pattern produced by primerb:
|
Combined typec | ||||
---|---|---|---|---|---|---|---|---|
A | B | C | D | E | ||||
1 | CBS 596.65 | Sugar; Louisiana (1965) | A-1 | B-1 | C-1 | D-1 | E-1 | A/D-1 |
3 | IHEM 1139 | Air; Brussels, Belgium (1982) | A-2 | B-2 | C-2 | D-2 | E-2 | A/D-2 |
4 | IHEM 9696 | Indoor air (hospital); Marseille, France (1991) | A-3 | B-3 | C-3 | D-3 | E-3 | A/D-3 |
5 | CBS 209.92 | Soils; La Palma, Spain (1992) | A-4 | B-4 | C-4 | D-4 | E-3 | A/D-4 |
6 | CBS 133.55 | Textile buried in soil; Utrecht, The Netherlands (1955) | A-5 | B-5 | C-5 | D-5 | E-4 | A/D-5 |
2 | CBS 102278 | Human, subcutaneous infection; Utrecht, The Netherlands (1999) | A-6 | B-6 | C-6 | D-6 | E-5 | A/D-6 |
7 | NCPF 2848 | Human, brain; Bristol, United Kingdom (1991) | A-3 | B-3 | C-3 | D-7 | E-3 | A/D-7 |
8 | NCPF 2951 | Human, sputum; Bristol, United Kingdom (1993) | A-3 | B-3 | C-3 | D-8 | E-3 | A/D-8 |
9 | NCPF 2922 | Human, ethmoid sinus; Bristol, United Kingdom (1993) | A-7 | B-3 | C-3 | D-3 | E-3 | A/D-9 |
10 | NCPF 2744 | Human, sputum; Bristol, United Kingdom (1990) | A-8 | B-7 | C-3 | D-9 | E-6 | A/D-10 |
11 | NCPF 2766 | Human, mitral valve prosthesis; Bristol, United Kingdom (1990) | A-9 | B-8 | C-3 | D-10 | E-3 | A/D-11 |
12 | AZN 677 | Bronchoalveolar lavage specimen, patient 1; University Medical Center, Nijmegen, The Netherlands (1992) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
13 | AZN 678 | Sputum, patient 1; University Medical Center, Nijmegen, The Netherlands (1992) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
14 | AZN 682 | Autopsy specimen, patient 1; University Medical Center, Nijmegen, The Netherlands (1992) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
15 | AZN 741 | Hematology ward, room; University Medical Center, Nijmegen, The Netherlands (1993) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
16 | AZN 924 | Hematology ward, room; University Medical Center, Nijmegen, The Netherlands (1993) | A-11 | B-10 | C-3 | D-12 | E-3 | A/D-13 |
17 | AZN 943 | Laboratory contaminant; University Medical Center, Nijmegen, The Netherlands (1993) | A-12 | B-10 | C-8 | D-13 | E-3 | A/D-14 |
18 | AZN 2725 | Sputum, patient 2; University Medical Center, Nijmegen, The Netherlands (1995) | A-13 | B-10 | C-8 | D-12 | E-3 | A/D-15 |
19 | AZN 3297 | Feces, patient 3; University Medical Center, Nijmegen, The Netherlands (1995) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
20 | AZN 6989 | Ascites fluid, patient 4; University Medical Center, Nijmegen, The Netherlands (1997) | A-11 | B-10 | C-3 | D-12 | E-3 | A/D-13 |
21 | AZN 7134 | Hematology ward, room; University Medical Center, Nijmegen, The Netherlands (1997) | A-10 | B-9 | C-7 | D-11 | E-3 | A/D-12 |
For a detailed description of isolates 12 to 21, see reference 14.
Numbers of different RAPD types for the following primers: A, 13; B, 10; C, 8; D, 13; E, 6.
Results of the combined analysis of the RAPD patterns produced by primers A and D. The number of different RAPD types for the combined type was 15.