Table 1.

Primers and settings for PCR recognition of T. gondii B1 gene

Primer	Sequence (5′- 3′)	Size	PCR conditions
First round (Outer primer)	F: GGAACTGCATCCGTTCATGAG	200 bp	95 °C for 5 min followed by 40 cycles (95 °C / 30 s, 56 °C / 30 s, 72 °C/30 sec)
	R: TCTTTAAAGCGTTCGTGGTC
Second round (Inner primer)	F: TGCATAGGTTGCAGTCACTG	100 bp	95 °C for 5 min followed by 35 cycles (95 °C / 30 s, 56 °C / 30 s, 72 °C / 30 s)
	R: GCGACCAATCTGCGAATACACC