Table 2. Median distances between the membrane and C-terminal end of the protein from smFRET experiments.

The distance values were extracted from the distributions shown in Figure 3b in the main text. The numbers in parentheses indicate the 95% confidence interval for experiments. Asterisk (*) indicates distance was beyond the FRET range for the snap surface 594 and Cy5 dye pair (Ro = 8.4 nm; Sisamakis et al., 2010).

Sample conditions	Distance (nm)
0% POPS, 100% POPC; -EGF	12.1 [11.6, 12.7]
0% POPS, 100% POPC; +EGF	8.8 [8.2, 9.6]
15% POPS, 85% POPC; -EGF	9.2 [8.8, 9.6]
15% POPS, 85% POPC; +EGF	11.9 [11.4, 12.4]
30% POPS, 70% POPC; -EGF	9.2 [9.1, 9.3]
30% POPS, 70% POPC; +EGF	*13.9 [12.5, 14.4]
60% POPS, 40% POPC; -EGF	11.4 [11.1, 11.7]
60% POPS, 40% POPC; +EGF	11.2 [10.9, 11.6]