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PCR amplification of C. difficile variant strains using tcdA primers NK9-NK11. Lanes: 1, strain 98-11522; 2, strain 98-15523; 3, strain 98-15845; 4, negative control; 5, ATCC 43596 (serotype C); 6, ATCC 43598 (serotype F); 7, strain 98-15632; 8, strain 98-16948; 9, strain 98-3050; 10, strain 98-4318; 11, strain 98-7426; 12, strain 60; λ, 100-bp ladder (Biolabs, Saint Quentin en Yvelines, France).
