FIG. 3.

Analysis of polypeptide and antigen expression in whole cells of the B. bronchiseptica clinical isolates. (A and B) Whole-cell lysates of the indicated strains were run in duplicate SDS-polyacrylamide gels and either stained with Coomassie brilliant blue (A) or transferred to polyvinylidene difluoride membranes for immunoblotting and probed with serum from a rat infected with B. bronchiseptica RB50 (B). The positions of the molecular weight markers (in thousands) are shown at the left. (C) Quantitation of total antigen expression by a whole-cell antigen-based ELISA. The same serum sample used for panel B was used as the primary antibody.