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Western immunoblot of RB50 whole-cell lysate antigen probed with serum from rats infected with isolate 222 or isolate 814. Lanes 1, 3, and 5, serum from different rats infected with isolate 222 for either 14 days (lanes 1 and 3) or 49 days (lane 5); lanes 2, 4 and 6, serum from different rats infected with isolate 814 for either 14 days (lanes 2 and 4) or 49 days (lane 6). The positions of the molecular weight markers (in thousands) are shown at the left.
