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. 2002 Jun;40(6):2153–2162. doi: 10.1128/JCM.40.6.2153-2162.2002

FIG. 5.

FIG. 5.

Analysis of clinical isolates. Multiplex PCR products were separated in a 2% agarose gel. M, 100-bp DNA ladder; (−), negative water control; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; 2 Templates, FOX-1 and ACT-1; C.o.(−), carryover negative control. (A) Lanes 1 to 4, 6 to 8, and 12, E. coli isolates; lanes 5 and 9, K. pneumoniae isolates; lane 10, P. mirabilis isolate; lane 11, E. aerogenes isolate. (B) Lanes 1, 2, 4, and 10, E. coli isolates; lanes 3 and 5 to 9, K. pneumoniae isolates. The amplified product from each PCR is indicated on the right, and the size of the marker in base pairs is shown on the left.