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. 2002 Sep;40(9):3455–3462. doi: 10.1128/JCM.40.9.3455-3462.2002

FIG. 1.

FIG. 1.

Some of the primers used to generate amplicons suitable for in vitro translation. Primer HCFLAG-3 is a sense primer containing an EcoRI restriction site upstream of a T7 promoter core sequence, followed by filler DNA (including three Gs immediately after the T7 core sequence) to allow for ribosomal scanning, a Kozak consensus sequence, a Flag tag (DYKDDDDK) coding region, and a region homologous to HCV sequence, in frame with the Flag coding region. Primer HCFLAG-4 is an antisense primer containing a stretch of 15 Ts to generate a poly(A) tail at the time of transcription, a stop codon in frame with a Flag coding region, and a region homologous to HCV sequence. Primer HCFLAGALA is a modified HCFLAG-3 with an additional codon encoding for alanine.