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. 2002 Sep;40(9):3455–3462. doi: 10.1128/JCM.40.9.3455-3462.2002

FIG. 3.

FIG. 3.

Mass spectrometry analysis of peptides translated from amplicons obtained with primers HCFLAGALA and HCFLAG-4. (A) Peptide obtained from template pCV-H77C. The predicted mass of the peptide, after methionine cleavage and N-terminal acetylation, is 7,102 Da. (B) Peptide obtained from template pCV-J4L6S. The predicted mass, after methionine cleavage and N-terminal acetylation, is 7,206 Da. (C) Peptides obtained from mixing the amplicons of pCV-H77C and pCV-J4L6S. (D) Peptides obtained from the H-77 serum aliquot.