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. 2002 Sep;40(9):3364–3373. doi: 10.1128/JCM.40.9.3364-3373.2002

FIG. 4.

FIG. 4.

Screen capture of F2 melting peak analysis, gel electrophoresis of amplicons, and sensitivity of the FRET probes specific for M. tuberculosis complex. Five copies of M. tuberculosis genome were reproducibly detected with probes with a melting peak of 64°C.