Figure 4.

The level of tel-eccDNA is decreased during development. Genomic DNA from Xenopus embryos and Xenopus erythrocytes was separated on a 2D gel: (A and B) 590 ng of early blastula embryos (pre-MBT, 1000-cell stage); (C and D) 1.4 μg of late neurula (90000 cells per embryo); (E and F) 1 μg of erythrocytes DNA. The quantity of embryonic DNA was estimated from the embryonic stage and checked by hybridization with a genomic DNA probe in comparison with DNA calibrated at 260 nm and loaded on the same gel (data not shown). The blots were hybridized with telomeric (A, C and E) and satellite 1 (B, D and F) probes.