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Kinetics of tel-eccDNA formation in vitro. (A) Sperm nuclei were incubated in egg extracts in the presence (open circles) or absence (solid squares) of 100 μg/ml aphidicolin. DNA replication kinetics was followed during a 2 h period by incorporation of [α-32P]dCTP. Samples were taken at the indicated times for 2D-gel analysis followed by hybridization with telomere probe (B).
