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. 2002 Oct;40(10):3586–3595. doi: 10.1128/JCM.40.10.3586-3595.2002

TABLE 2.

Antibiotic resistance profiles of nosocomial E. coli isolates

Isolate Genetic typea Antibiotic resistance profileb intI Antibiotic resistance genesc
Class 1 integron
Cephamycinase
flo
Cassette(s) size (kb) Genes blaCMY2 Molecular size (kb) of fragment generated by PFGEd
39737 1A ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri + 1.7, 0.80 aadA5, dfrA17 + −
22-2 1A ESCp, Chl, Flq, Spc, Tet I, Sul, Tri, Flor + 1.7, 0.90 aadA5, dfrA17 + −
29 1A ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri + 1.7, 0.60 aadA5, dfrA17 + 25 −
34 1A ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.7, 0.90 aadA5, dfrA17 + −
53 1A ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri + 1.5, 0.75 + −
56 1A ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor I + 1.5, 0.75 + −
22949 1B ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 1.4, 0.70 + +
4479 1B ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 1.3, 0.75 + +
38 1B ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 1.2, 0.60 + 185 +
4517A 2 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.7, 0.75 aadA5, dfrA17 + 250 +
4517B 2 ESCp, Chl, Flq, Spc, Tet, Sul, Tri, Flor + 1.7, 0.75 aadA5, dfrA17 + +
22255 2 ESCp, Chl, Flq, Spc, Tet, Sul, Tri, Flor + 0 + +
A1-72 3 ESCp, Chl I, Flq, Spc I, Sul, Gen, Flor, Tet − 0 + 100 +
1745 3 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 0.90 + −
32 3 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.7, 0.90 aadA5, dfr17 + −
27315 4 ESCp, Chl, Flq, Spc I, Tet, Flor + 0 + 140 +
40362 5 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor − 0 + +
42614B 6 Sul − 0 − 280, 115 −
1888 7 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.7, 1.1, 0.75 aadA5, dfrA17 + 30, 27 +
18813 7 ESCp, Chl, Flor, Amk I + 0 + +
22559 8 ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 1.4, 0.70 + +
25055a 8 ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 0 + +
25055b 8 ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 1.3, 0.70 + +
29610 9 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.3, 0.70 + +
12279 9 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.2, 0.60 − +
21 10 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor I + 1.7, 0.90 aadA5, dfr17 + 29 −
42614A 11 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor − 0 + +
18397 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 2.4, 1.2 + +
23120 ESCp, Chl, Flq, Spc I, Tet, Gen, Sul, Tri, Flor + 0 + +
21411 ESCp, Chl, Flq, Spc, Tet, Gen, Sul, Tri, Flor + 1.4, 0.70 + +
37 ESCp, Chl, Flq, Spc, Tet, Sul, Tri + 0 + −
55-1 ESCp, Chl, Flq, Tet, Sul, Tri, Flor I + 0 + −
55-2 ESCp, Chl, Flq, Tet, Sul, Tri, Flor I + 0 + −
55-3 ESCp, Chl, Flq, Spc I, Tet, Sul, Tri, Flor + 0 + −
a

A numerical designation was assigned to each distinct DNA fingerprint generated by PFGE with both restriction enzymes XbaI and BlnI. Isolates with same number have identical PFGE patterns with either restriction enzyme.

b

ESCp, extended-spectrum cephalosporin resistance, including resistance to the cephalosporins (cefazolin, cefoxitin, cephalothin and ceftioufur), β-lactams (amoxicillin, ampicillin and ticarcillin), and the β-lactam inhibitor clavulanic acid; Flq, fluoroquinolones (enrofloxacin and orbifloxacin). The bacterial isolates were also resistant to chloramphenicol (Chl), gentamicin (Gen) spectinomycin (Spc), sulfonamides (Sul), tetracycline (Tet), and trimethoprim (Tri).

c

Bacterial isolates were screened for the presence of class 1 integrons by PCR by using the integrase gene intI as a marker for this genetic element. A second PCR was done with primers specific for conserved sequences flanking the integron integration site aatI. This PCR amplifies a gene cassette(s) present within aatI (32). PCR amplicons were sequenced, and the nucleotide or translated amino acid sequences were queried against the GenBank DNA database at the National Center for Biotechnology Information for matches. The genes and the corresponding integron cassette size(s) are highlighted in boldface. Other antibiotic resistance genes for which the isolates were surveyed in this study included blaCMY2 (67) and flo (27).

d

Genetic mapping of blaCMY2 was done for canine E. coli isolates representative of the different genetic types by DNA-DNA hybridization of PFGE generated with restriction endonuclease XbaI and hybridized with PCR-generated DNA probe specific for blaCMY2. The molecular sezis of XbaI DNA fragment(s) that hybridized with blaCMY2 probe are given.