TABLE 1.
Primers used for PCR and sequencing and species-specific probes used for dot blot hybridization
| Primer (reference) | Primer sequence | Positiona (direction) | Target geneb |
|---|---|---|---|
| CAT1 (2) | 5′-GATTCAATTGGTTTGAA(G/A)GAGGCT-3′ | 1181-1204 (→) | htrA |
| CAT2 (2) | 5′-TCACATCACCAGG(A/G)CGTATTC-3′ | 1578-1598 (←) | htrA |
| PAPn1 (44) | 5′-TTCTAGGAGTTGAAACCGAT-3′ | 437-456 (→) | pap31 |
| PAPn2 (44) | 5′-GAAACACCACCAGCAACATA-3′ | 694-713 (←) | pap31 |
| 536F (20) | 5′-CAGCAGCCGCGGTAATAC-3′ | 536-553 (→) | 16S rDNA |
| 1050R (20) | 5′-CACGAGCTGACGACA-3′ | 1050-1035 (←) | 16S rDNA |
| RH1 (1) | 5′-GGTGCGTTAATTACCGATCC-3′ | 1208-1227 (→) | htrA |
| RQ1 (1) | 5′-GGCGCTTTGATTACTGATCC-3′ | 1208-1227 (→) | htrA |
a
Arrows indicate direction of primers (→, forward; ←, reverse).