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Discriminant metabolites identified in fetal rat brain (GD21) following in utero exposure to PTU (a) or PCN (b). Metabolites were selected based on PLS-DA VIP scores and retained only when annotation was confirmed at level 2 of the MSI. Heatmaps display relative peak intensities (green = low, red = high) across control and treated groups, accompanied by fold-change values relative to controls. N = 10 per group
