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Restriction enzyme assay profile of EHV-2 isolates and reference strains. EHV-2 isolates (E1 and E2), EHV-2 strains H40, T400, and LK4, and EHV-5 strain P48, were amplified in RK-13 cell monolayer. Viral DNA was purified by Genomic-tip 20/G column, digested with Hind III and Eco RI and analysed by gel electrophoresis. DNA marker φX-174 Hae III and λ-RF/Hind III is seen in lane M.
