TABLE 2.
Genotyping of N. meningitides clones by direct sequencing and the MLST-DHPLC methoda
| Locus fragment | No. of clones with different genotypes by direct sequencingb | No. of clones with different genotypes by MLST-DHPLCc | No. of isolates of the following genotype by sequencing/MLST-DHPLC
|
||
|---|---|---|---|---|---|
| Serogroup Cd | Serogroup Be | Serogroup Wf | |||
| fumC | 11 | 11 | 1/1 | 9/9 | 1/1 |
| pgm | 9 | 9 | 1/1 | 7/7 | 1/1 |
| gdh | 9 | 9 | 1/1 | 7/7 | 1/1 |
| pdhc | 8 | 8 | 1/1g | 7/7 | 1/1 |
| adk | 7 | 7 | 2/2 | 5/5 | 1/1h |
| aroE | 19 | 19 | 1/1 | 17/17 | 1/1 |
| abc | 19 | 19 | 1/1 | 17/17 | 1/1 |
a
The 39 clones were screened for seven of the loci evaluated by MLST. For each locus, the number of different genotypes per among isolates of serogroup C, B, or W are indicated.
b
Number of clones with variations in sequences.
c
Number of clones with differences in chromatogram profiles.
d
Of 8 strains tested.
e
Of 29 strains tested.
f
Of two strains tested.
g
The sequence of one of the serogroup B genotype isolates was identical to the serogroup C sequence.
h
The sequence of one of the serogroup B genotype isolates was identical to the serogroup W sequence.