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. 2005 Nov 16;1:11. doi: 10.1186/1746-4811-1-11

Table 1.

Detergents successfully employed for the solubilization of protein complexes prior to BN-PAGE.

Detergent Sample Detergent concentration Reference
Dodecylmaltoside Mammalian 1.1% [1]
Mitochondria
Plant Mitochondria 1.5% [103]
Chloroplasts 2.0% [75]
Yeast Peroxisomes 0.5% and 1.0% [81]

Digitonin Mammalian 4 g/g protein [104]
Mitochondria
Plant Mitochondria 5.0 g/g protein [6]
Chloroplasts 1.5 g/g protein [41]
Yeast Microsomes 1.0% [80]
Yeast Peroxisomes 1.0% [81]

Triton X-100 Mammalian 1.0, 1.4 and 2.4% [105]
Mitochondria
Plant Mitochondria 0.5% [6]
Yeast Peroxisomes 0.2% and 1.0% [81]
Whole cellular lysates of different human cell lines 0.1% [3]

Brij 96 Whole cellular lysates of different human cell lines 0.5% [3]

Octylglucoside Tobacco microsomes 40 mM [2]

Saponin Whole cellular lysates of different human cell lines 1.0% [3]