TABLE 2.
Primers used in this study
| Primer set | Primer | Sequence (5′ to 3′) | Target gene | Location within gene | Size of PCR amplicon (bp) | GenBank accession no. |
|---|---|---|---|---|---|---|
| A | Stx1-a | TCTCAGTGGGCGTTCTTATG | stx1 | 777-796 | 338 | M17358 |
| Stx1-b | TACCCCCTCAACTGCTAATA | 1114-1095 | ||||
| Stx2f-a | TGTCTTCAGCATCTTATGCAG | stx2f | 300-320 | 150 | M29153 | |
| Stx2f-b | CATGATTAATTACTGAAACAGAAAC | 449-425 | ||||
| Stx2-a | GCGGTTTTATTTGCATTAGC | stx2 | 1228-1247 | 115 | X07865 | |
| Stx2-b | TCCCGTCAACCTTCACTGTA | 1342-1323 | ||||
| B | Stx2c-a | GCGGTTTTATTTGCATTAGT | stx2c | 1186-1205 | 124 | M59432 |
| Stx2c-b | AGTACTCTTTTCCGGCCACT | 1309-1290 | ||||
| Stx2e-a | ATGAAGTGTATATTGTTAAAGTGGA | stx2e | 204-228 | 303 | M36727 | |
| Stx2e-b | AGCCACATATAAATTATTTCGT | 506-485 | ||||
| EAE-a | ATGCTTAGTGCTGGTTTAGG | eaeA | 132-151 | 248 | Z11541 | |
| EAE-b | GCCTTCATCATTTCGCTTTC | 379-360 | ||||
| C | Stx2d-a | GGTAAAATTGAGTTCTCTAAGTAT | stx2d | 1221-1244 | 175 | AF043627 |
| Stx2d-b | CAGCAAATCCTGAACCTGACG | 1395-1375 | ||||
| HlyA-a | AGCTGCAAGTGCGGGTCTG | EHEC hlyA | 867-885 | 569 | X79839 | |
| HlyA-b | TACGGGTTATGCCTGCAAGTTCAC | 1435-1412 | ||||
| RfbE-a | CTACAGGTGAAGGTGGAATGG | rfbEO157 | 673-693 | 327 | S83460 | |
| RfbE-b | ATTCCTCTCTTTCCTCTGCGG | 999-979 | ||||
| FliC-a | TACCATCGCAAAAGCAACTCC | fliCH7 | 1068-1088 | 247 | AF228488 | |
| FliC-b | GTCGGCAACGTTAGTGATACC | 1314-1294 | ||||
| Alla | E16S-a | CCCCCTGGACGAAGACTGAC | E. coli 16S rRNA | 1682-1701 | 401 | AB035924 |
| E16S-b | ACCGCTGGCAACAAAGGATA | 2082-2063 |
a
Used in all sets as the internal control.