TABLE 3.
Major virulence genes detected by the three primer sets in the multiplex PCR analysis
| Toxin(s) confirmed by PCR or PCR-RFLP analysis (n)a | Serotype (n)b | No. of genes detected by PCR with primer set:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| A
|
B
|
C
|
|||||||||
| stx1 | stx2 | stx2f | stx2c | stx2e | eaeA | stx2d | hlyA | rfbEO157 | fliCH7 | ||
| Stx1 (13) | Non-O157:non-H7 (5) | 5 | —c | — | — | — | 4 | — | 5 | — | — |
| O111:NM (1) | 1 | — | — | — | — | 1 | — | 1 | — | — | |
| O91:NM (1) | 1 | — | — | — | — | — | — | 1 | — | — | |
| O157:H7 (5) | 5 | — | — | — | — | 5 | — | 5 | 5 | 5 | |
| O157:NM (1) | 1 | — | — | — | — | 1 | — | 1 | 1 | 1 | |
| Stx1 + Stx2 (27) | O UT:H UTd (1) | 1 | 1 | — | — | — | — | — | — | — | — |
| O157:H7 (26) | 26 | 26 | — | — | — | 26 | — | 26 | 26 | 26 | |
| Stx1 + Stx2c (2) | O157:H7 (1) | 1 | — | — | 1 | — | 1 | — | 1 | 1 | 1 |
| O111:NM (1) | 1 | — | — | 1 | — | 1 | — | — | — | — | |
| Stx1 + Stx2d (3) | O128:H? (2) | 2 | — | — | — | — | — | 2 | 2 | — | — |
| O rough:NM (1) | 1 | — | — | — | — | — | 1 | — | — | — | |
| Stx2 (16) | O157: H7 (16) | — | 16 | — | — | — | 16 | — | 16 | 16 | 16 |
| Stx2 + Stx2c (27) | O157:NM (1) | — | 1 | — | 1 | — | 1 | — | 1 | 1 | 1 |
| O157:H7 (26) | — | 26 | — | 26 | — | 26 | — | 26 | 26 | 26 | |
| Stx2c (10) | O91:H21 (1) | — | — | — | 1 | — | — | — | 1 | — | — |
| Non-O157:non-H7 (3) | — | — | — | 3 | — | — | — | 1 | — | — | |
| O157:H7 (5) | — | — | — | 5 | — | 5 | — | 5 | 5 | 5 | |
| O55:H7 (1) | — | — | — | 1 | — | 1 | — | — | — | 1 | |
| Stx2d (1) | O UT:H8 (1) | — | — | — | — | — | — | 1 | — | — | — |
| Stx2e (1) | O139:K82 (1) | — | — | — | — | 1 | — | — | — | — | — |
| Stx2f (1) | O128:B12 (1) | — | — | 1 | — | — | 1 | — | — | — | — |
| Stx negative (28) | O157:non-H7 (7) | — | — | — | — | — | 6 | — | — | 7 | — |
| O157:NM (3) | — | — | — | — | — | — | — | — | 3 | — | |
| Non-O157:H7 (11) | — | — | — | — | — | 1 | — | — | — | 11 | |
| O UT:H7 (3) | — | — | — | — | — | — | — | — | — | 3 | |
| Non-O157:NM (2) | — | — | — | — | — | — | — | — | — | 1 | |
| Non-O157:non-H7 (1) | — | — | — | — | — | — | — | — | — | — | |
| O UT:H UT (1) | — | — | — | — | — | — | — | — | — | — | |
| Total | 101/129 | 45 | 70 | 1 | 39 | 1 | 96 | 4 | 92 | 91 | 106 |