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. 2005 Nov 28;102(49):17699–17704. doi: 10.1073/pnas.0502679102

Fig. 1.

Fig. 1.

Zebrafish NCX1 homologues. (A) Phylogeny of NCX homologues in rat, mouse, canine, trout, and zebrafish. The tree was calculated in clustalx by using the neighbor-joining method. Numbers at branches are bootstrap values. The unit of the scale represents amino acid substitutions per site. (B) Both NCX1n and NCX1h share high identity to other NCX1 proteins throughout the entire peptide. (C–E) In situ hybridization analysis showed that NCX1n is expressed throughout the zebrafish embryo at 16S (C) and becomes more strongly expressed in the brain and the neural tube at 24 hpf (D). By 48 hpf, NCX1n expression is restricted to the brain, the neural tube, and the fin bud, but not in the heart (E). Inset in E shows the ventral view focusing on the heart. The arrow points to the heart. (F–H) NCX1h is a cardiac-specific gene. NCX1h is expressed in cardiac precursors at 16S (F). At 24 hpf, the transcripts of NCX1h are detected throughout the primitive heart tube (G). By 48 hpf, NCX1h transcripts are found prominently in the myocardium of the atrium and ventricle and are barely detectable in the outflow tract (H). a, atrium; v, ventricle; o, outflow tract. Arrows point to the heart.