Fig. 2.

Typical CSX methylation patterns. Each tag is arranged in a 5′ to 3′ horizontal order, with open circles representing unmethylated CpG sites and filled circles representing methylated sites. Eight tags are sampled from each gland. There are eight glands and a total of 64 tags per uterus. Tags are different within and between glands in the same uterus. Below is the 3′ region of the CSX gene, illustrating primer locations (underlined) and CpG sites in the CSX tag (red) and CSX6 tag (yellow). The CSX6 tag has a G/T polymorphism that distinguishes alleles in heterozygous individuals.