Fig. 3.

The DV fails to close in Ahr^fx/fxCre^Tek mice. (A) Representative examples of livers from Ahr^fx/fx, Ahr^fx/fxCre^Alb, and Ahr^fx/fxCre^Tek mice perfused with trypan blue. Livers were cannulated via the portal vein, flushed with PBS, and then injected with 0.5 ml of trypan blue. Upon perfusion of a normal liver with trypan blue, complete filling of the hepatic vasculature resulted in a blue coloration and indicated normal DV closure. In contrast, failure of the liver to turn blue indicated porto-systemic shunting and a patent DV. M, median lobe; L, left lobe. Representative angiographs of livers (B) and frequency of DV patency (C) in adult Ahr^fx/fx, Ahr^fx/fxCre^Alb, and Ahr^fx/fxCre^Tek mice.