Fig. 4.

Adaptive metabolism and hepatocellular toxicity are dependent on AHR signaling in hepatocytes. Mice were treated once per week for 4 wk with 100 μg of dioxin/kg in DMSO or DMSO alone and then killed 7 d after the final injection. White bars, vehicle-treated animals; gray bars, dioxin-treated animals. The Ahr^fx/fxCre^Alb groups each contain eight animals, whereas the vehicle-treated Ahr^fx/fx group contains four animals, and the dioxin-treated Ahr^fx/fx group contains 12 animals. Error bars, SE. Those groups not sharing a superscript letter differ significantly at P ≤ 0.05. (A) Liver weights in Ahr^fx/fx and Ahr^fx/fxCre^Alb mice in response to dioxin treatment. (B) Representative Northern blot analysis of Cyp1a1, Cyp1a2, and Cyp1b1 expression in Ahr^fx/fx and Ahr^fx/fxCre^Alb mice in response to dioxin treatment. Gapdh expression was used as a loading control. (C) ALT values in Ahr^fx/fx and Ahr^fx/fxCre^Alb in response to dioxin treatment.