Table 1. Rate constants, control coefficients, and activation energies used in the model calculations.
| Reaction i | Rate constant ki, h-1 | CiP (15% variation of the kis) | Ei, kJ/mol | CiPEi, kJ/mol |
|---|---|---|---|---|
| 1 | 0.3 | 0.131 | 190 | 24.9 |
| 2 | 0.3 | 0.093 | 190 | 17.7 |
| 3 | 0.3 | -0.040 | 190 | -7.6 |
| 4 | 0.27 | -0.470 | 30 | -14.1 |
| 5 | 0.2* | -0.131 | 30† | -3.9 |
| 6 | 0.2* | -0.628 | 30† | -18.8 |
| ΣiCiP = -1.045 | ΣiCiPEi = -1.8 |
The given rate constant values are defined for Tref = 292 K. Rate constants k5 and k6 (with asterisk) differ for the various frq mutants. Initial concentrations (a.u.) used in all calculations: X = 6.124 × 10-2, Y = 8.452 × 10-2, Z = 5.245 × 10-1. Threshold for frq transcription inhibition, Zmax = 0.1 a.u.; threshold for reactivating frq transcription, Zmin = 0.05 a.u. (used in all calculations).
k5, k6 values for frq+ at Tref = 292 K. The following k5, k6 values (defined at Tref = 292 K) have been used in the calculations for the other mutants: frq1, 0.320 h-1; frq7, 0.124 h-1; frqS5131, 0.080 h-1.
E5 and E6 values for frq+. The following E5, E6 values (E5 = E6) have been used in the calculations for the other mutants (see also Table 2): frq1, 29 kJ/mol; frq7, 46 kJ/mol; frqS5131, 59 kJ/mol.