Fig. 4.
mtDNA deletions of the muscle and brain of the Twinkledup mouse, determined by long PCR, cloning, and DNA sequencing. (a) Schematic diagram of mouse mtDNA showing the deleted segment in gray (see supporting information for details). Only the D-loop, 12S rRNA, and 16S rRNA were commonly spared. (b) Schematic presentation of the mouse and human mtDNA region harboring the 5′ deletion breakpoints. Black vertical lines show the 5′ deletion breakpoints identified in this study, and the asterisks below indicate how often the same 5′ breakpoint was found from dissimilar deletion molecules. The common 5′deletion breakpoint of human multiple mtDNA deletions (16070) is indicated.