TABLE 2.
Comparison of real-time TaqMan PCR and enterovirus PCR on part of the third EU-QCCA enterovirus proficiency panel
| Virus stock | Virus titer (TCID50)a | Real-time TaqMan PCR result | In-house nested PCR result | % Positive PCR result (n = 82)b |
|---|---|---|---|---|
| CVA9 | 0.036 | Negative | Negative | 17 |
| CVA9 | 0.36 | Positive | Equivocal | 51 |
| CVA9 | 3.6 | Positive | Positive | 87 |
| EV11 | 25.2 | Positive | Equivocal | 72 |
| EV11 | 252 | Positive | Positive | 87 |
| EV11 | 25.200 | Positive | Positive | 95 |
a
TCID50, 50% tissue culture infectious dose.
b
n = the number of participants.