Skip to main content
. 2005 Dec 8;102(51):18572–18577. doi: 10.1073/pnas.0509239102

Fig. 3.

Fig. 3.

Effects of the repression of genes encoding individual subunits on complex formation. (A) Cells were infected with vA16i in the absence (-A16) or presence (+A16) of IPTG or with vG9i in the absence (-G9) or presence (+G9) of IPTG or with vLacOI and transfected with a plasmid expressing H2-V5 controlled by its native promoter. NP40-soluble extracts were incubated with immobilized antibody to V5 (lanes 1–6), and the bound proteins were analyzed by Western blotting with monoclonal antibody to V5 (H2-V5) or with polyclonal antibodies to A21, A28, and L5 as indicated. As a control, 10% of the NP40-soluble extracts were analyzed directly by Western blotting (lanes 7–10). (B) Cells were infected with vA21i in the absence (-A21) or presence (+A21) of IPTG or with vLacOI and transfected with a plasmid expressing H2-V5 protein controlled by its native promoter. NP40-soluble extracts of infected cells were incubated with immobilized antibody to V5, and the bound proteins were analyzed as in A.