Fig. 1.

Spheroid formation using the MicoCell plate and the final syringe product.

(A) Photograph of the 42,000-cavity MicoCell plate; approximately 2.1 × 10⁷ ADSCs are seeded to form 42,000 spheroids (500 cells/spheroid).

(B) Microscopic images illustrating spheroid formation within 72 h (top: NIBS minipig; bottom: patient).

(C) Representative syringe containing the final S-ADSC suspension (42,000 spheroids in 5 mL Ringer’s lactate) immediately before intra-articular injection; spheroids are visible as fine floating particulates.