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In vivo and in vitro P. carinii f. sp. hominis organisms. (a and b) Parasites in a fresh bronchoalveolar lavage fluid sample stained with ortho-toluidine blue (a) or methanol-Giemsa (b) stains. (c and d) Clustered vegetative and cystic forms attached to L2 epithelial alveolar cells in short-term culture after 24 h (c) or 48 h (d). Arrows, Pneumocystis cysts containing well-visible intracystic bodies; arrowheads, trophozoites. Bar, 10 μm.
